Bufflo when Inseminated please tell me On oestrus Mid oestrus or end of the oestrus or when eve

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morning as observed estrus, between noon and 6 p.m. on the day of observed estrus, or cows bred the following morning after observed estrus the previous evening. This indicates that a single mid-morning insemination for all cows observed in estrus the night before or the same morning should yield acceptable conception.
In Buffalo heat period is longer then cow some times 30to 40hrs so multial AI is recomended with the interval of 12 hrs
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Most animal won’t show the sign, better is two kmsemination from date of identification for two consecutive days , use same bull Semen
End of oestrus mainly ... Or mid of oestrus.... Ok Sir??
Thank you doctor
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End of mid oestrus...i.e.after 12hrs of starting oestrus
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After the end of esterus in both
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Late/End of oestrus
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End of oestrus
Thank you doctor
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